Suppressed-Priming PCR, a Novel Concept of DNA Quantification Based on PCR Kinetics
نویسندگان
چکیده
منابع مشابه
Suppressed-priming PCR, a novel concept of DNA quantification based on PCR kinetics.
The concept of an unbiased DNA quantification method is proposed based on PCR kinetics. A set of PCRs was performed with a dilution series of primers, which differently limited DNA amplification. The difference in amplification efficiency between the DNA templates reflected the estimates of their relative amounts. This concept might serve as a theoretical base for further development of accurat...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2009
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.90266